By Fraser Armstrong, Julea Butt, Jacques Breton, Andrew J. Thomson (auth.), Milton J. Allen, Stephen F. Cleary, Arthur E. Sowers, Donald D. Shillady (eds.)
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Additional resources for Charge and Field Effects in Biosystems—3
For x-ray diffraction analysis, oriented phosphOlipid multilayers were prepared by placing a small drop of lipid/chloroform solution on a flat piece of aluminum foil and evaporating the chloroform under a stream of nitrogen. , 1987). The specimen was then mounted in a controlled humidity chamber on a single-mirror (line-focussed) x-ray camera such that the x-ray beam was oriented at a grazing angie relative to the multilayers on the foil. The humidity chamber consisted of a copper canister with two mylar windows for passage of the x-ray beam.
The number of 12 molecules adsorbed, per phospholipid molecule, was determined using the optical techniques described previously. Fig. 2 displays the electrical conductivity of EPC and DPPC films, respectively, as a function of the number of 12 molecules adsorbed by the films, per phospholipid molecule. The data points represent the average of measurements from a minimum of three different films in each case. The steady-state value of the electrical current at each adsorbed iodine value was attained within 48 hours.
This degree of surface coverage is intended to allow the oxidase to parti tion into the layer. , 1987). , 1989) . Following the octadecyl mercaptan modification a cholate dialysis is performed. , 1972; Zhang, 1984; Zhang, 1985). Briefly, cholate preventp amphiphiles from forming vesicles. As the cholate is removed from such solutions via dialysis, vesicles form and the enzyme incorporates unidirectionally into the vesicle bilayers (Casey, 1984). The aim here is to use these ideas to prepare bilayers on gold electrodes with cytochrome oxidase partitioned into these structures unidirectionally.
Charge and Field Effects in Biosystems—3 by Fraser Armstrong, Julea Butt, Jacques Breton, Andrew J. Thomson (auth.), Milton J. Allen, Stephen F. Cleary, Arthur E. Sowers, Donald D. Shillady (eds.)