By Simon P. Langdon
Specialist researchers describe intimately their most efficient and updated equipment for transforming into melanoma cells within the laboratory. there are ways to signify and authenticate phone traces, to isolate and advance particular forms of melanoma cells, and to strengthen new mobile line types. sensible assays are supplied to guage clonogenicity, mobile proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and mobile cycle parameters. different equipment enable the amendment of melanoma cells for transfection, improvement of drug resistance, immortalization, and move in vivo; the co-culture of alternative mobile varieties; and the detection and remedy of infection.
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Additional info for Cancer Cell Culture: Methods and Protocols (Methods in Molecular Medicine)
Similarly, if a cell line is maintained for only a limited period of time in culture (for example, 10 passages) before replacement with fresh stocks, then phenotypic drift should be minimal. References 1. O’Brien, S. J. (2000) Cell culture forensics. Proc. Natl. Acad. Sci. USA 98, 7656–7658. 2. Stacey, G. , Masters, J. R. , Hay, R. , Drexler, H. , Macleod, R. A. , and Freshney, R. I. (2000) Cell contamination leads to inaccurate data: we must take action now. Nature 403, 356. 3. Macleod, R. A. , Dirks, W.
Tally Counter. Syringe and needles or Pasteur pipets. Microscope. Hemocytometer (Improved Neubauer). 4% Trypan blue in PBS (optional). 10% Formalin (optional). 2. Electronic Counting 1. 2. 3. 4. As above but with electronic counter, for example, Beckmann Coulter Z2. Counting pots. Fixed volume dispenser (optional). Ice tray. 6. For Cryopreservation 1. 2. 3. 4. 5. 6. 7. 8. 9. Cells requiring freezing. Cell culture medium: Medium plus 10% FCS. Freezing mixture: 10% DMSO/20% serum/70% medium or 10% DMSO/90% serum.
Natl. Acad. Sci. USA 98, 7656–7658. 2. Stacey, G. , Masters, J. R. , Hay, R. , Drexler, H. , Macleod, R. A. , and Freshney, R. I. (2000) Cell contamination leads to inaccurate data: we must take action now. Nature 403, 356. 3. Macleod, R. A. , Dirks, W. , and Drexler, H. G. (2002) Persistent use of misidentified cell lines and its prevention. Genes Chromosomes Cancer 33, 103–105. 4. Gartler, S. M. (1968) Apparent HeLa contamination of human heterodiploid cell lines. Nature 217, 750–751. 5. Nelson-Rees, W.
Cancer Cell Culture: Methods and Protocols (Methods in Molecular Medicine) by Simon P. Langdon